![]() ![]() She says she doesn't know what she would have done if they didn't come. Masha was finally rescued at the age of 10. So once I said something - I said everything else. "It was like, I finally had someone to talk to. Holtz remembers her asking: "Is this about my secret?" An agent had taken Mancuso inside his house, but even there, he tried to keep Masha from talking by yelling out to her.īut Masha spoke anyway. The agents found computer disks with child pornography - but the biggest discovery was to come from Masha herself. "He wasn't happy that we were there and it was obvious to us," he said. They immediately separated them, and Clinton says he could tell Mancuso was concerned. When they pulled up, they saw Mancuso and Masha outside. On May 27, 2003, federal agents Denise Holtz and Tom Clinton visited Mancuso's home, looking for the photos advertised on the Internet. Zaglifa pretended to want them too, and struck up a conversation with someone using the handle "NkdSister." After chatting with him for a bit, he had a gut feeling - and traced NkdSister's Internet address to Pittsburgh. There is a limited supply of child porn, and so pedophiles are always looking for arousing new images - so when Masha's fresh pictures appeared, this caused a feeding frenzy. Mike Zaglifa had been posing as a pedophile on the Internet, where obscene pictures of children are often traded like baseball cards. The road to Masha's rescue began hundreds of miles away in the Chicago suburb of Palos Heights. So I just didn't tell anybody, 'cause I was afraid." "He wouldn't tell me what it would be, but he'd just say something bad would happen. "He'd tell me not to tell anyone, or else something bad would happen," Masha said. To keep her silent he used rewards - as well as threats. ![]() "But it always came back to me - couldn't stop it." ![]() The Charles River " hairless" rat mutation is distinct from the hairless mouse alleles."I'd make myself think of other things when it was happening," she said. The Charles River (CR) " hairless" rat is one of the autosomal recessive hypotrichotic animal models actively studied in pharmacologic and dermatologic research. Despite its widespread use, the molecular basis of this monogenic mutation remains unknown, and the skin histologic features of this phenotype have never been described. However, the designation " hairless" has been used as an extension of the hairless mouse (hr) nomenclature on the basis of the clinical absence of hairs in both phenotypes. We present a description of the histopathologic changes in heterozygous and homozygous CR hairless rat mutants during the first month of life. The postnatal homozygous rat skin was characterized by abnormal keratinization of the hair shaft and formation of a thick and dense layer of corneocytes in the lower portion of the epidermal stratum corneum. This layer prevented the improperly keratinized hair shaft from penetrating the skin surface. Starting from the latest stages of hair follicle (HF) development, obvious signs of HF degeneration were observed in homozygous skin. This process was extremely rapid, and by day 12, mainly atrophic HFs with abnormal or broken hairs were present in the skin. Therefore, the mutation in the CR rat abrogates cell proliferation in the hair matrix and affects keratinocyte differentiation in the HF and interfollicular epidermis, a phenotype that is completely distinct from hr/hr. To test whether the CR rat harbored a mutation in the hr gene, we analyzed the coding region of this gene and consensus intron splice site sequences in mutant rats and found no mutation, further supporting phenotypic evidence that the hairless phenotype in CR rats is not allelic with hairless. ![]()
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